Journal: Cancer immunology, immunotherapy : CII
Article Title: Receptor desensitization and blockade of the suppressive effects of prostaglandin E 2 and adenosine on the cytotoxic activity of human melanoma-infiltrating T lymphocytes
doi: 10.1007/s00262-010-0924-z
Figure Lengend Snippet: Effect of CADO and PGE2 on cAMP production (a) and CREB (b) and Csk (c) phosphorylation. a TILs #2009 at stage II were incubated with CADO (50 μM) and/or PGE2 (0.5 μM) or CGS (50 μM) and/or butaprost (5 μM) for 10 min. The levels of cAMP in cellular extracts were analyzed using mass spectrometry. b, c TILs #2009 at stage II were stimulated with anti-CD3 in the presence of CADO (50 μM) and/or PGE2 (0.5 μM) for 30 min. TIL protein extracts (50 μg) were resolved using 10% SDS-PAGE and transferred to PVDF membranes. The level of CREB (b) and Csk (c) phosphorylation was analyzed using specific antibodies. The optic density (OD) ratio of the bands was analyzed using ImageQuanT data analysis software (Molecular Dynamics)
Article Snippet: Films were scanned and analyzed by ImageQuanT data analysis software (Molecular Dynamics) [ 17 ].
Techniques: Phospho-proteomics, Incubation, Mass Spectrometry, SDS Page, Software